MEDSI2018 - List of Authors (Perez-Berna, A.J.)

Paper	Title	Page
THPH43	New Holder for Dual-Axis Cryo Soft X-Ray Tomography of Cells at the Mistral Beamline	427
	R. Valcárcel, C. Colldelram, N. Gonzalez, E. Pereiro, A.J. Pérez-Berná, A. Sorrentino ALBA-CELLS Synchrotron, Cerdanyola del Vallès, Spain
	A new dual-axis sample holder has been designed and built for the Transmission soft X-ray Microscope (TXM) at the MISTRAL beamline (ALBA Synchrotron) to perform cryo-soft X-ray tomography of cells with dual tilt configuration to reduce the missing wedge. The design, with restricted dimensions Ø7x30mm, enables using commercial Auto-Grid support rings that give rigidity to the sample grid handling. It consists of a guided miniature handle with a spring system that allows sample rotation by 90° around the beam axis inside vacuum and in cryogenic conditions by using the TXM sample loading robot keeping a rotation of ±65° at the sample stage. Two magnets fix the positions at 0° and 90°.The two tilt series can be collected consecutively and the use of Au fiducials permits combining both improving the final quality of the 3D reconstructions. In particular, cellular features hidden due to their orientation with respect to the axis of rotation become visible. The main frame is made in aluminium bronze to enhance the thermal conductivity and in addition, all the pieces have undergone an ion implantation treatment in order to reduce friction and improve the anti-seizure property of the parts.
DOI •	reference for this paper ※ https://doi.org/10.18429/JACoW-MEDSI2018-THPH43
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Paper

Title

Page

New Holder for Dual-Axis Cryo Soft X-Ray Tomography of Cells at the Mistral Beamline

427

R. Valcárcel, C. Colldelram, N. Gonzalez, E. Pereiro, A.J. Pérez-Berná, A. Sorrentino
ALBA-CELLS Synchrotron, Cerdanyola del Vallès, Spain

A new dual-axis sample holder has been designed and built for the Transmission soft X-ray Microscope (TXM) at the MISTRAL beamline (ALBA Synchrotron) to perform cryo-soft X-ray tomography of cells with dual tilt configuration to reduce the missing wedge. The design, with restricted dimensions Ø7x30mm, enables using commercial Auto-Grid support rings that give rigidity to the sample grid handling. It consists of a guided miniature handle with a spring system that allows sample rotation by 90° around the beam axis inside vacuum and in cryogenic conditions by using the TXM sample loading robot keeping a rotation of ±65° at the sample stage. Two magnets fix the positions at 0° and 90°.The two tilt series can be collected consecutively and the use of Au fiducials permits combining both improving the final quality of the 3D reconstructions. In particular, cellular features hidden due to their orientation with respect to the axis of rotation become visible. The main frame is made in aluminium bronze to enhance the thermal conductivity and in addition, all the pieces have undergone an ion implantation treatment in order to reduce friction and improve the anti-seizure property of the parts.

DOI •

reference for this paper ※ https://doi.org/10.18429/JACoW-MEDSI2018-THPH43

Export •

reference for this paper using ※ BibTeX, ※ LaTeX, ※ Text/Word, ※ RIS, ※ EndNote (xml)